昆虫肠道真菌分离及活性初探
摘 要
本文简要综述和讨论目前已经报道的昆虫肠道真菌分离鉴定概况，了解肠道来源真菌在发现结构新颖的活性次级代谢产物中的前景。课题拟从10-20种昆虫中（以在土壤、腐殖质或者植物生存的昆虫或者某个发育阶段为主），重点关注幼虫或者成虫的肠道中的真菌，并进行昆虫中肠道真菌的分离、鉴定以及初步的活性筛选，期望发现具有生物活性（如对杂草生长或其种子萌发有抑制作用）的真菌菌株，并进行保藏，为开展活性次级代谢产物的分析、鉴定提供宝贵的菌株资源，具有十分重要的研究与开发价值。
研究内容主要包括：
- 昆虫在不同发育阶段的个体收集，拍照，鉴定。
- 解剖个体发现肠道，刮取肠道壁等方式获得微生物菌群。
- 稀释微生物菌群、涂布平板、挑取较纯单菌落进而分离纯化出真菌的纯净单菌落。
- 根据生长形态以及显微镜观察对真菌进行初步的形态学鉴定，并通过薄层色谱或高效液相指纹色谱进行分子生物学鉴定，最终拍照保存。
- 真菌菌株资源的保藏。
- 对某一些微生物进行小量的发酵培养并开展抗杂草真菌活性。
关键词：昆虫；肠道微生物；次级代谢产物；发酵培养
Isolation of intestinal fungi from insects
ABSTRACT
In this paper, the isolation and identification of insect intestinal fungi, which have been reported at present, are briefly reviewed and discussed, so as to understand the prospect of intestinal fungi in discovering active secondary metabolites with novel structures. From 10-20 kinds of insects (mainly insects living in soil, humus or plants or at a certain development stage), the subject plans to focus on the fungi in the intestinal tract of larvae or adults, and to separate, identify and screen the intestinal fungi in insects, hoping to find and preserve fungal strains with biological activity (such as inhibiting the growth of weeds or their seed germination), providing valuable strain resources for the analysis and identification of active secondary metabolites.The research contents mainly include:
（1） Collecting, photographing and identifying individuals of insects at different developmental stages.
以上是毕业论文文献综述，课题毕业论文、任务书、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
