拟南芥突变体max4的鉴定与表型观察文献综述

 2022-04-14 09:04

拟南芥max2突变体经激活标签农杆菌转化后的表型筛选文献综述

摘要:独脚金内酯(Strigolactones,SLs)作为一类新型植物生长调节因子,因最早发现于独脚金属植物而得名,后又见于列当属植物。独脚金内酯是萜类化合物、醇类化合物及人工合成类似物的总称,其分离与生物学功能是当今植物学研究热点。独脚金内酯对植物分枝有重要调控功能,尽管MAX2是独脚金内酯在植物发育中的信号转导的重要元件,本实验拟利用携带激活标签载体的农杆菌转化max2突变体,筛选其表型回复为野生型的转化植株,由此克隆相应被激活的基因,从而鉴定拟南芥中独脚金内酯信号转导途径可能的新作用元件。

关键词:独脚金内酯,植物分枝,生物学功能,激活标签,拟南芥

A review of the literature on phenotypic screening of Arabidopsis max2 mutants after transformation by agrobacterium activator

Abstract: Strigolactones (SLs), as a new class of plant growth regulators, was first found in the cyclopean metal plants and was later found in the genus Taxon. Witchetonolactone is a general term for terpenoids, alcohols and synthetic analogues, and its isolation and biological function is a hot topic in botany research. Witchweed lactone branch has important control functions to the plant, although MAX2 witchweed lactone is an important component of signal transduction in plant development, this study intends to use to carry active label carrier of agrobacterium-mediated transformation max2 mutants, screening the phenotypic response for the conversion of wild type plants, the corresponding activated genes, cloning and identification of arabidopsis thaliana witchweed lactone in signal transduction pathways may be the new role of the components.

Keywords: Solodactone, plant branching, biological function, activation label, Arabidopsis.

独脚金(Striga asiatica O.K.)又名独脚柑、消米虫,属于玄参科植物,主要分布于我国云南、广西、贵州以及湖南西部等地,独脚金内酯最早发现于独脚金非寄主植物棉花根际分泌物中(Cook et al.,1966;Cook et al.,1972),为独脚金醇(Strigol);1998年又在小列当四叶草中提取到独脚金醇的同分异构体--列当醇(Orobanchol)(Yokota et al.,1998);随后,又相继从独脚金的寄主高粱(Sorghum bicolor)、玉米(Zeamays)和小米(Pennisetum glaucum)中分离得到独脚金醇(周晓燕,2016)。研究发现,独脚金内酯对一些寄生植物,如独脚金属、列当属和黑蒴属等的种子萌发具有促进作用(Hauck et al.,1992;Yokota et al.,1998;Xie X et al,2009;Xie XN et al.,2010;);同时,还发现独脚金内酯可刺激丛枝真菌菌丝分枝以及调节植物分枝生长等多种功能和作用,参与调节植物生长发育和形态建成,其分离合成、化学成分、调控途经与作用机理以及生物学功能成为植物学研究的热点问题,且已经取得重大进展。

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