比率以及比色型次氯酸盐荧光探针及其在活细胞、细菌和斑马鱼生物成像中的应用
摘 要
次氯酸（HClO）/次氯酸盐（ClO－）是活性氧（ROS）的重要组成部分，在人体免疫系统中起着重要作用。HClO/ClO－能破坏侵袭性细菌和病原体，在低浓度下就可以调节生物体的生理平衡，引起生物细胞内蛋白质、胆固醇、核酸等生物分子的氧化，导致一系列疾病。因此，对生物体内HClO/ClO－的含量进行定量分析已迫在眉睫。本文设计合成了香豆素-水杨酸肼-席夫碱（CMSH），以香豆素为荧光单元的比色荧光探针。结果表明，CMSH对克隆鉴定具有较高的选择性和敏感性。此外，比率（I470/I532）分别在40 s和128 nm的限制范围内显示出出色的ClO依赖性快速和敏感性能。溶液颜色也由绿色变为无色，伴随着加入，荧光从绿色变成蓝色。CMSH已成功地应用于活体细胞、细菌和斑马鱼的成像，具有细胞毒性低、渗透性好等特点。
关键词：荧光探针、比色法、次氯酸/次氯酸盐、活细胞生物成像、细菌和斑马鱼。
Ratiometric and colorimetric fluorescent probe for hypochlorite monitor and application for bioimaging in living cells, bacteria and zebrafish
ABSTRACT
Hypochlorous acid ()/hypochlorite () was a biologically important component of reactive oxygen species (ROS) and plays a key role in human immune function systems. /can destroy invasive bacteria and pathogens, and mediate the physiological balance of the organism with low concentrations, and cause oxidation of the biomolecules such as proteins, cholesterol and nucleic acid in biological cells, leading to a series of diseases with over capacity. Therefore, quantifying the content of / in organisms are extremely urgent. In this work, coumarin-salicylic hydrazide Schiff base (CMSH), a ratiometric and colorimetric fluorescent probe for detection based on coumarin as the fluorophore unit was rationally designed and synthesized. The results indicated that CMSH exhibits high selectivity and sensitivity for identification. Additionally, the ratios (I470/I532) displayed brilliant -dependent quick and sensitive performance within 40 s and limitation of 128 nm, respectively. As well as the color of the solution changes from green to colorless accompanied by the fluorescence form green turns into blue with addition of . Totally, CMSH has been successfully employed as ratiometric sensor to image in living cells, bacteria and zebrafish with low cytotoxicity and good permeability.
Key words：Fluorescent probe, Ratiometric and colorimetric, Hypochlorous acid/hypochlorite, Bioimaging in living cells, Bacteria and zebrafish.
1介绍
以上是毕业论文文献综述，课题毕业论文、任务书、外文翻译、程序设计、图纸设计等资料可联系客服协助查找。
